Igcr1
Web24 jun. 2024 · According to the actual model, IGCR1 seems to work as an insulator to delimit Eμ action/function. These findings are contradicted by Busslinger’s group who … Web11 feb. 2024 · Here we report that in the absence of intergenic control region 1 (IGCR1) the intronic enhancer (Eµ) associates with the next available CTCF binding site located close to VH81X, via putative heterotypic interactions involving YY1 and CTCF. Figure 1 and S3 show that Eµ loops to an alternate site close to the 3’-most functional VH gene segment, …
Igcr1
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WebFunctionally, IGCR1 uses CTCF looping/insulator factor-binding elements and, correspondingly, mediates Igh loops containing distant enhancers. IGCR1 promotes normal B-cell development and balances antibody repertoires by inhibiting transcription and rearrangement of D H-proximal V H gene segments and promoting rearrangement of … Web5 apr. 2024 · To test the interpretation that increased V H 81X gene transcription on IGCR1-mutated alleles resulted from association with Eμ, we used CRISPR/Cas9 to delete Eμ sequences from a pro-B cell line with IGCR1-mutated IgH alleles. We obtained two independent lines in which Eμ was deleted on both alleles in the context of a mutated …
Web11 feb. 2024 · Figure 2 and S4 support that Eu-VH81X loop requires both YY1 and CTCF. Figure 3 and S5 demonstrate that DFL16.1 utilization is reduced on IGCR1-deficient alleles. Figure 4 and S6 shown that VH (VH713 and VHQ52) recombination is greatly induced and Eu-dependent on IGCR1-deficient alleles. Figure S7 show the verification of IGCR1 … WebDownload scientific diagram IGCR1 mediates long-distance Igh chromosomal loops. a, Schematic of chromosome interactions between IGCR1-containing and 3′ Igh CBE …
Web9 sep. 2011 · IGCR1 promotes normal B cell development and balances antibody repertoires by inhibiting transcription and rearrangement of D H-proximal V H s and … WebDownload scientific diagram RAG1 and RAG2 Recruitment to WT and IGCR1-Mutated Alleles (A) Scale schematic of the IgH locus indicating V H gene families (J558/3609, mid, and 7183/Q52) and the 3 0 ...
WebDownload scientific diagram IGCR1 mutations alter VH usage, germline transcription and rearrangement order. a, PCR analyses of indicated VH family rearrangements in pro-B …
Web11 sep. 2011 · IGCR1 maintains ordered and lineage-specific VH(D)JH recombination by suppressing VH joining to D segments not joined to JH segments, and VH to DJH joins in thymocytes, respectively. the hub ajaxWeb1 jul. 2024 · Intergenic control region 1 (IGCR1), which contains 2 CTCF sites, lies just upstream of the distal D segment DFL16.1 (refs 82,83), and a cluster of 10 CTCF sites … the hub airedaleWebTo examine possible function of IGCR1, we have generated porcine aortic endothelial (PAE) cells over-expressing IGCR1. We demonstrate that IGCR1 expression in PAE cells inhibited cell proliferation and capillary tube formation as measured by colorimetric MTT and matrigel tube formation assays, respectively. In contrast, over-expression of IGCR1 ... the hub agencyWebATAC peaks in the E -DQ52 and 3′ part of the locus were unchanged by the IGCR1 mutation (Fig. 1C, black boxes), whereas peaks corresponding to IGCR1 were absent on mutated alleles (Fig. 1C, red ... the hub aiken techWeb11 sep. 2011 · Functionally, IGCR1 uses CTCF looping/insulator factor-binding elements and, correspondingly, mediates Igh loops containing distant enhancers. IGCR1 promotes … the hub akcWeb8 sep. 2016 · When IGCR1 is inactivated, V H 81X is used in the vast majority of V H to DJ H rearrangements, despite integrity of the remaining IgH locus (Guo et al., 2011; Hu et al., 2015). Based on these observations, we hypothesized that, in place of V H 81X, the human IGHV1-2*02 would similarly dominate V H usage in the context of IGCRI deletion. the hub albertonWeb28 jun. 2024 · IGCR1 deletion extends this recombination tracking domain directionally upstream of the DJ H RC to the proximal V H s, coupled with dramatically increased proximal V H to DJ H joining, most dominantly V H 81X (Hu et al., 2015). However, the nature of the tracked substrate and factors that drive RAG tracking remained speculative. the hub alabang